October 9 - 12, 2012
Building 10 & Natcher Conference Center
- General Schedule of Events
- Opening Plenary Session
- Concurrent Symposia Sessions
- Poster Sessions
- National Graduate Student Research Conference
- FARE Award Ceremony
- Special Exhibits on Resources for Intramural Research
- TSA Research Festival Exhibit Show
- Clinical Center Tours
- Research Festival Committees
- Past Research Festivals
Identification and elimination of immunodominant T cell epitope in a recombinant immunotoxin used to treat cancer
| Friday, November 08, 2013 — Poster Session IV | |||
|---|---|---|---|
2:00 p.m. – 4:00 p.m. |
FAES Academic Center (Upper-Level Terrace) |
NCI |
IMMUNO-17 |
Authors
- R. Mazor
- J. Eberle
- A. Vassall
- R. Beers
- X. Hu
- I. Benhar
- I. Pastan
Abstract
Moxetumomab pasudotox and SS1P are immunotoxins that have been developed in our lab to treat cancer. This talk described identification and elimination of T cell epitopes in the bacterial moiety of immunotoxins. We used an vitro stimulation step with the whole protein which specifically expands T-cell populations that are reactive to naturally processed peptide, followed by ELISpot. This strategy had a 100% response rate which correlates with the immunogenicity response rate of patients that have been treated with immunotoxin. We identified one highly-reactive and immunodominant epitope that stood out among multiple subdominant epitopes and constructed mutant RITs that retained cytotoxic activity, and had reduced T-cell immunogenicity.
