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The NICHD Zebrafish Core at One Year: a Focus on Reverse Genetics

Friday, November 08, 2013 — Poster Session III

10:00 a.m. – 12:00 p.m.

FAES Academic Center (Upper-Level Terrace)




  • B Feldman


The NICHD Zebrafish Core's goal is to help researchers of any expertise perform zebrafish experiments aimed at illuminating basic biology and human disease mechanisms, thereby advancing the NIH and NICHD 's research missions. A typical research question might investigate the function or regulation of a zebrafish gene and/or its ortholog from humans or another species. We offer consultation for any zebrafish-related projects you may be considering. The core is equipped to assist with the following methodologies: In situ hybridization to characterize developmental expression patterns of zebrafish genes; Zebrafish nucleic acid collection for downstream genomic and molecular applications; Transgenic animal production; design and microinjection of antisense morpholino oligonucleotides for transient loss of function; design, synthesis and microinjection of TALE nucleases or CRISPR-Cas9 reagents to generate germ-line genetic mutants; cryopreservation of transgenic and mutant zebrafish lines; importation of existing zebrafish lines; characterization of phenotypes by time-lapse photography.

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