October 9 - 12, 2012
Building 10 & Natcher Conference Center
- General Schedule of Events
- Opening Plenary Session
- Concurrent Symposia Sessions
- Poster Sessions
- National Graduate Student Research Conference
- FARE Award Ceremony
- Special Exhibits on Resources for Intramural Research
- TSA Research Festival Exhibit Show
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- Past Research Festivals
HOST GENES REQUIRED FOR INTEGRATION OF Tf1, A LONG TERMINAL REPEAT (LTR) RETROTRANPOSON OF SCHIZOSACCHAROMYCES POMBE
| Friday, November 08, 2013 — Poster Session III | |||
|---|---|---|---|
10:00 a.m. – 12:00 p.m. |
FAES Academic Center (Upper-Level Terrace) |
NICHD |
GEN-29 |
Authors
- S Rai
- Henry Levin
Abstract
Long-terminal repeat (LTR) retrotransposons have complex modes of mobility in host genomes. The transcripts encode Gag, PR, RT and IN. RT then reverse transcribes the RNA into double-stranded cDNA and IN inserts this cDNA into new positions in the host genome. The limited coding capacity of retrotransposons necessitates an extensive reliance on host co-factors. Our lab studies integration of the LTR retrotransposon Tf1 from Schizosaccharomyces Pombe. In a broad effort to identify new host factors that are associated with Tf1 integration, I am screening a collection of 3004 strains of S. pombe containing deletions of non-essential genes. Screening results of the first 2,393 strains identified 208 strains have reduced transposition. Results from a genetic assay that measures homologous recombination of Tf1 cDNA indicate that 119 of the 208 strains with reduced transposition have a defect in the process of integration. Identified factors are such as Rhp18 that play an important role in DNA repair, proteins that mediate transcription, signal transduction and protein synthesis; Fep1 encodes an iron-responsive transcriptional repressor; pht1 (Htz1 in budding yeast) histone variant H2AZ, exchanges for histone H2A in nucleosomes. The role of these proteins in integration at promoters will be tested.
