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Genome-wide RNAi screening at the NIH

Friday, November 08, 2013 — Poster Session III

10:00 a.m. – 12:00 p.m.

FAES Academic Center (Upper-Level Terrace)




  • S.E. Martin
  • E. Buehler
  • Y. Chen
  • C. Klumpp-Thomas
  • P. Ormanoglu


Gene silencing through RNAi has emerged as a powerful tool for understanding gene function. Over the past several years, high-throughput RNAi screens have illuminated a wide variety of biology ranging from genes that affect the activity of therapeutic agents to elucidating novel components of signaling pathways. The NIH has recently established a state-of-the-art RNAi screening facility that conducts large-scale RNAi screening projects in collaboration with NIH investigators. The facility assists with all stages of projects beginning with assay development through genome-wide siRNA screens, informatics/pathway analysis, and rigorous follow-up. Considerable emphasis has also been placed on establishing a robust computational infrastructure, as the goal of the facility is to provide collaborating investigators with highly enriched and validated lead genes. Druggable genome and genome-wide siRNA screens for both human and mouse are available, and miRNA mimic and inhibitor libraries are routinely included in screens. An overview of the facility as well as some example applications will be presented.

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