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Unbiased Segregation of Fission Yeast Chromosome 2 Strands to Daughter Cells

Friday, November 08, 2013 — Poster Session III

10:00 a.m. – 12:00 p.m.

FAES Academic Center (Upper-Level Terrace)




  • A Klar
  • M Bonaduce


Location of DNA strands with respect to each other at the centromere is fixed. Thereby, directed segregation occurs to specific daughter chromatid copies in eukaryotic cells. We determined whether the cylindrical-shaped yeast cell distributes the specific chromosomal strand to the same cellular pole in successive cycles of cell division. By observing the pattern of recurrent mating-type switching in progenies of individual cells by microscopic analyses, we found that chromosome 2 strands are distributed by the random mode in successive cell divisions. We also exploited unusual “hotspot” recombination features of this system to investigate whether there is selective segregation of strands such that oldest Watson-containing strands co-segregate in the diploid cell at mitosis. Our genetic data suggest that chromosome 2 strands are segregated independently to those of the homologous chromosome.

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