October 9 - 12, 2012
Building 10 & Natcher Conference Center
- General Schedule of Events
- Opening Plenary Session
- Concurrent Symposia Sessions
- Poster Sessions
- National Graduate Student Research Conference
- FARE Award Ceremony
- Special Exhibits on Resources for Intramural Research
- TSA Research Festival Exhibit Show
- Clinical Center Tours
- Research Festival Committees
- Past Research Festivals
BLAST+: Powerful Applications for Sequence Analysis
| Friday, November 08, 2013 — Poster Session IV | |||
|---|---|---|---|
2:00 p.m. – 4:00 p.m. |
FAES Academic Center (Upper-Level Terrace) |
NLM |
COMPBIO-2 |
Authors
- G.M. Boratyn
- C. Camacho
- A. Fong
- N. Ma
- T.L. Madden
Abstract
Basic Local Alignment Search Tool (BLAST) is one of the more popular methods for searching sequence databases. The stand-alone BLAST+ applications allow users to run a variety of different searches on their own hardware. These range from nucleotide-nucleotide comparisons with megaBLAST to searches that employ a Position Specific Scoring Matrix with DELTA-BLAST and PSI-BLAST. We describe the stand-alone BLAST+ applications and discuss some features that users are often not aware of. These include the ability to save search strategies for future execution as well as the ability to save search results in a structured format so that multiple BLAST reports (e.g., tabular or XML) can be produced at a later date upon demand. We also discuss the database masking feature of the BLAST+ suite which can mask regions (e.g., simple and interspersed repeats) in the database sequences. Finally, we also describe the wide variety of reports that BLAST+ suite can produce and discuss possible future improvements.
