October 9 - 12, 2012
Building 10 & Natcher Conference Center
- General Schedule of Events
- Opening Plenary Session
- Concurrent Symposia Sessions
- Poster Sessions
- National Graduate Student Research Conference
- FARE Award Ceremony
- Special Exhibits on Resources for Intramural Research
- TSA Research Festival Exhibit Show
- Clinical Center Tours
- Research Festival Committees
- Past Research Festivals
Extranuclear DNA: A Normal Phenomenon or an Indication of Pathology?
| Thursday, November 07, 2013 — Poster Session II | |||
|---|---|---|---|
12:00 p.m. – 2:00 p.m. |
FAES Academic Center (Upper-Level Terrace) |
NEI |
CHEMCELL-19 |
Authors
- AJ Ogilvy
- DF Shen
- Y Wang
- CC Chan
- M Abu-Asab
Abstract
The appearance of extranuclear DNA (enDNA) in cells is a phenomenon that has been known for years, but has only recently received attention in the literature. The objective of our investigation was to determine whether or not the appearance of extranuclear DNA in the RPE layer of the mouse eye was a normal phenomenon, or due to a pathological state. We analyzed four different strains of mice using electron microscopy, using both ultrastructural analysis and immunolabeling techniques. In degenerate RPE cells we found presence of enDNA, which was confirmed by immunolabeling, but no enDNA in healthy RPE cells. This suggests that enDNA is the product of some pathological event inside the cell that causes chromatin material to leak out of the nucleus and into the cytoplasm.
