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Multimodality longitudinal imaging of tissue remodeling in a murine hindlimb ischemia model

Thursday, November 07, 2013 — Poster Session II

12:00 p.m. – 2:00 p.m.

FAES Academic Center (Upper-Level Terrace)




  • Z Sun
  • G Tong
  • L Lang
  • G Niu
  • X Chen


The tissue ischemia can lead to serial complicated pathophysiological processes, including tissue necrosis, inflammation, angiogenesis, tissue remodeling and regeneration. In this study, we applied multimodality longitudinal imaging to map several key pathophysiological processes in a real-time and non-invasive manner. Hindlimb ischemia was induced by ligation and excision of the left femoral artery. Longitudinal PET imaging was performed from day 1 to day 21 after surgery with several tracers. 18F-albumin PET was applied to evaluate limb blood perfusion and vascular permeability. 18F-FDG PET was used to evaluate tissue inflammation and 18F-Alfatide to evaluate limb angiogenesis. Optical imaging using an MMP activatable probe was employed to evaluate MPP activity post hindlimb ischemia. With imaging, the highest vascular permeability was observed at 24 h after surgery as indicated by high local uptake of 18F-albumin at 2 h p.i.. Local accumulation of 18F-FDG reached the maximum at day 3 after surgery. Both 18F-Alfatide and MMP activity showed the highest level at day 7 after surgery. Thus, with noninvasive multimodality longitudinal imaging, we successfully mapped the kinetics of the comprehensive pathophysiological changes of hindlimb ischemia in a surgical mouse model.

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