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Real time monitoring of cellular response to targeted therapy using quartz crystal microbalance with dissipation monitoring

Thursday, November 07, 2013 — Poster Session II

12:00 p.m. – 2:00 p.m.

FAES Academic Center (Upper-Level Terrace)




  • BV Chikkaveeraiah
  • A Bhirde
  • A Jin
  • X Chen


The quartz crystal microbalance with dissipation monitoring (QCM-D) is an ultrasensitive, label-free technique allowing real time measurement of changes in frequency, and energy dissipation of a layer attached on to the quartz surface. Changes in frequency indicate the changes in mass, while changes in dissipation indicate changes in the viscoelastic character of the layer. Numerous nanoformulations are developed for therapeutic applications and multidrug resistance is a big challenge for successful chemotherapy. Gold nanoparticles have been used extensively to design the nanoformulation for targeting tumor cells which yield high affinity and specificity for target cells. CD44 is a tumor initiating glycoprotein that is overexpressed in many cancer cells making it a viable drug target. We employed QCM-D to monitor the responses of the drug resistant cancer cells towards the gold nanostars formulation targeting the CD44-overexpressing cancer cells. Hyaluronic acid (HA), a natural ligand for CD-44 was used for the nanoformulation. The frequency and dissipation changes of the tumor cells for the uptake of nanofomulations were monitored. Targeted Photothermal therapy employing gold nanostar formulation was efficient in counteracting the resistance of tumor cells.

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