|
|
|
|
|
Mini-Symposia
Session IV
Thursday,
October 4
|
Vaccination
Against Infectious Diseases
Chaired by John Mascola, VRC/NIAID
2:00 p.m. to 3:30 p.m.
Natcher
Conference Center
Main Auditorium
Recent
progress in our understanding of the
mechanisms of protective immunity
has begun to be translated into improved
methods of inducing immune responses
by vaccination. This knowledge has
been applied to diseases for which
no vaccine yet exists and to the potential
optimization of existing vaccines.
This session will focus on new knowledge
and vaccine strategies related to
development of protective vaccines
against a diverse group of infectious
diseases.
| Program |
|
| Nancy
Sullivan, VRC, NIAID |
Vaccination
Against Ebola |
| John
Robbins, NICHD |
Pertussis
Vaccines: Not All Virulence Factors are Protective
Antigens |
| Carole
Long, LPD, NIAID |
Progress Toward Development
of a Malaria Vaccine |
| John
Mascola, VRC, NIAID |
DNA and Viral Vectored HIV
Vaccine Candidates |
|
Reactive Oxygen Species: Roles
in Normal Physiologic Function
and in Disease
Chaired by Toren Finkel, NHLBI, and Sue Goo Rhee,
NHLBI
2:00 p.m. to 3:30 p.m.
Natcher
Conference Center
Balcony A
Cells
are constantly being exposed to reactive oxygen species
(ROS). A large body of experimental evidence suggests
that in many cases, a correlation exists between the
degree of oxidative stress and the progression of
a number of human diseases and conditions ranging
from aging to neurodegeneration. Although it is clear
that ROS can in certain situations function as random
and deleterious molecules, emerging evidence suggests
that a number of normal physiological processes are
also modulated by cellular redox conditions. As more
is learned about the role of oxidants in normal signaling
pathways, the concept of ROS in disease is undergoing
a healthy reevaluation. The purpose of this symposium
is to provide an update on the role of ROS as regulators
of a variety of physiological and pathophysiological
pathways. It is hoped that these presentations will
in turn provide the framework and perspective to understand
how ROS participate in disease progression.
| Program |
|
| Rod
Levine, NHLBI |
Oxidation
of Proteins and Control of Proteolysis |
| Dan
Sullivan, NHLBI |
In
Situ Identification of Proteins that Contain Redox
Active Cysteines |
| Tom
Leto, NIAID |
Possible Physiological and
Pathological Roles of NADPH Oxidases |
| Tong-Shin
Chang, NHLBI |
Regulation of Peroxiredoxin
I Peroxidase Activity by Cdc2 Kinase-dependent
Phosphorylation |
| Chang
C Chiueh, NIMH |
Different Role of OH and NO
in Animal Models of Parkinsonism |
|
Nanobiology
Chaired by Kuan Wang, NIAMS
2:00 p.m. to 3:30 p.m.
Natcher
Conference Center
Balcony B
Recent
advances in nanotechnology have led to the rapid development
of promising analytical tools that can observe, probe
and manipulate single molecules and single organelles
in aqueous environments. The objective of this mini-symposium
is to explore the state of the art methods that are
used to understand the behavior of individual macromolecules
and assembles and their interactions in real time.
The
session will be tutorial in style and delivered by
practicing intramural experts. The basic principles
of single molecule fluorescence methods, laser tweezers
and atomic force microscopy will be emphasized as
practical tools to detect motion, rotation and force
of biological motors, folding/unfolding of elastic
proteins, order and strength of interactions of nucleosome
components and membrane phase separation. Emerging
trends of nanobiology will also be discussed.
| Program |
|
| Kuan
Wang, NIAMS |
Nanobiology:
An Era of Swinging Singles |
| Michael
Lewis, NIAMS and Kuan Wang, NIAMS |
Single
Molecule Fluorescence Microscopy: Seeing is Believing |
| James
Sellers, NHLBI |
Laser Tweezers:
Light Fantastic |
| Jeffery
Forbes, Albert Jin, OD, and Kuan Wang, NIAMS |
Molecular Force
Spectroscopy: Stretching Proteins Beyond Their
Means |
| Sanford
Leuba. NCI |
Laser Tweezers:
Teasing Nucleosomes on a Single Chromatin Fiber |
| James
A. Dvorak, NIAID, Fuyuki Tokumasu, NIAID, and
Albert Jin, OD |
Atomic Force Microscopy:
Seeing Waves in a Lipid Sea |
|
The
Liver as Target and Regulator of Immune Responses
to Infectious Pathogens
Chaired by Thomas A. Wynn, NIAID and Barbara Rehermann,
NIDDK
2:00 p.m. to 3:30 p.m.
Natcher
Conference Center
Balcony C
The
liver is an immunologically distinct
organ that contains its own unique
population of cells of the innate
(NK, NKT cells) and adaptive (CD4
and CD8 T cell) cellular immune response.
It has been proposed that the liver
is the site of induction of immune
responses (J Immunol 2001; 166:5430-5438)
as well as the site of elimination
of apoptotic immune cells (J Immunol
2001; 166:3035-3041). This dual function
is important to understand the pathogenesis
of liver disease caused by parasitic
and viral pathogens and to understand
inductionof tolerance upon exposure
to oral and allograft antigens. This
mini-symposium will address the unique
role of the liver as the target and
regulator of cellular immune responses
to infectious pathogens from different
research perspectives to enhance discussion
and collaborations.
| Program |
|
| Jens
Bukh, NIAID |
Molecular
Biology and Pathogenesis of Viral Hepatitis C |
| Barbara
Rehermann, NIDDK |
Cellular
immune responses to viral liver pathogens |
| Denise
L. Doolan, Naval Medical Research Center |
Induction and Characterization
of Liver-stage Protective Immunity Against Malaria |
| Matthias
Hesse, NIAID |
Role of Arginase-1
in Parasite-induced Liver Fibrosis |
|
Mitochondria
and Apoptosis
Chaired by Richard Youle, NINDS,
and Steve Zullo, NIST/NCI
2:00 p.m. to 3:30 p.m.
Natcher
Conference Center
Conference Room E1/E2
Apoptosis,
a physiological process of cell destruction, is key
to mammalian development, tissue turnover and cancer
prevention. Surprisingly, mitochondria and their associated
proteins initiate and regulate apoptosis. This symposium
will cover how the Bcl-2 family member Bax specifically
triggers a mitochondrial driven cascade of cell death,
how ion channels participate in neuronal apoptosis
and how mitochondria fission and fusion machinery
can regulate cell viability.
| Program |
|
| Annette
Khaled, NCI |
Bax
and Mitochondrial Injury: The Multiple Pathways
to Death Induced by IL-7 Withdrawal |
| Mark
Mattson, NIA |
Anti-Apoptotic
Functions of Potassium Channels in Neurons |
| Stephan
Frank, NINDS |
The Role of Dynamin-Related
Protein 1, a Mediator of Mitochondrial Fission,
in Apoptosis |
|
Bacteriophage
at the NIH: An Early Genetic Model Expands Disease
Treatment and Diagnosis
Chaired by Carl R. Merril, NIMH,
and Steve Zullo, NIST/NCI
2:00 p.m. to 3:30 p.m.
Natcher
Conference Center
Conference Room F1/F2
Bacteriophage
played a central role in the origins of molecular
biology studies at the NIH. These efforts have resulted
in advances that have provided a body of knowledge,
which is facilitating the application of bacteriophage
to a number of biomedical problems. The applications
that will be discussed in this mini-symposium range
from: the use of phage to study and enhance mutagenesis,
to the use of phage display for the study of protein-protein
interactions, and efforts to use phage as an antibacterial
therapeutic agent.
| Program |
|
| Sankar
Adhya, NCI |
Brief
Introduction and Overview of the History of Phage
Research at the NIH |
| Donald
L. Court, NCI |
High
Efficiency Mutagenesis, Using Double and Single-stranded
DNA |
| Alasdair
Steven, NIAMS |
Use of Phage Display
for the Study of Structural Biology |
| Susan
Garges and Glenn Merlino (NCI) |
The Use of Mice
Transgenic for Bacteriophage lambda: Inherent
Genetic Instability Associated with ErbB2-induced
Mammary Ttumorigenesis |
| Carl
R. Merril and Dean Scholl (NIMH) |
Use of Phage in
Antibacterial Therapy |
|
|
|
|
 |
