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Mini-Symposia
Session I
Wednesday,
October 3
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Molecular
Imaging
Chaired by Robert S. Balaban, NHLBI
11:00 a.m. to 12:30 p.m.
Natcher
Conference Center
Main Auditorium
This
symposium is devoted to the discussion of molecular
imaging in basic molecular biology as well as clinical
medicine. The scope of molecular imaging from cells
to animal models to clinical conditions will be reviewed.
Techniques including magnetic resonance, confocal
microscopy, and radioactive isotope approaches will
be highlighted.
| Program |
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| Alan
Koretsky, NINDS |
Anatomical,
Functional and Molecular MRI of the Brain |
| Jennifer
Lippincott-Schwartz, NICHD |
Analysis
of Secretory Protein Trafficking in Living Cells |
| Ronald
Neumann, CC |
Pinpointing Proteins with Positrons |
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Contemporary
Flow Cytometry: Analysis of Lymphocyte Function
Chaired by Mario Roederer, VRC/NIAID
11:00 a.m. to 12:30 p.m.
Natcher
Conference Center
Balcony A
The
power of flow cytometry to resolve
heterogeneity in the immune system
is unique. The most prevalent application
of flow cytometry has been to resolve
leukocyte subsets based on cell surface
protein expression patterns. However,
more researchers are turning to this
technology to provide detailed information
about the functional repertoire of
cells. This session will cover several
different types of functional measurements
made by flow cytometry that provide
a much more detailed understanding
of the roles of various cell types
in immune function--combining the
power of immunophenotyping to identify
distinct subsets together with functional
measurements to provide a cell-by-cell
analysis of the immune system.
| Program |
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| Calman
Prussin, NIAID |
High
Fidelity Analysis of T cell Cytokine Responses
Using Intracellular Staining |
| Stephen
De Rosa, VRC/NIAID |
Subsets
of gamma delta T cells Identified
and Functionally Characterized
Using 11-color Flow Cytometry |
| Ronald
Rabin, CBER/FDA |
Use of Flow Cytometry to Measure
Responses to Chemokines |
| Richard
Siegel, NIAMS |
FRET with GFP's: Visualizing
Protein-Protein Interactions with Flow Cytometry |
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Mitosis
and Meiosis
Sponsored by the Cell Cycle Interest Group
Chaired by Mary Lilly, NICHD, and Orna Cohen-Fix,
NIDDK
11:00 a.m. to 12:30 p.m.
Natcher
Conference Center
Balcony B
The mitotic and meiotic divisions
present many similar challenges of the cell. During
both divisions multiple events must be coordinated
to insure accurate chromosome segregation and division
of cellular components. The failure to do so may lead
an imbalance in the chromosome number as observed
in Down Syndrome and certain types of cancer. This
session will focus on the processes that govern progression
through meiosis and mitosis, including chromosome
cohesion and condensation, spindle dynamics and checkpoint
control. Both shared and unique features of meiosis
and mitosis will be discussed.
| Program |
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| Michael Lichten,
NCI |
Starting and
Finishing Meiotic Recombination |
| Mel Depamphillis,
NICHD |
Regulation
of Eukaryotic DNA Replication
through the Cell Cycle Dependent
Binding and Release of Origin
Recognition Proteins |
| Alexander
Strunnikov, NICHD |
Cohesin,
Condensin and Mitotic Chromosome
Structure |
| Monica
Murakami, NCI |
Cell Cycle
Regulation is Required for Normal
Gastrulation in Vertebrates |
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Mouse
Embryogenomics: Frontiers of Developmental Biology
and
Genomics
Chaired by Minoru Ko, NIA, and Colin Stewart, NCI
11:00 a.m. to 12:30 p.m.
Natcher
Conference Center
Balcony C
The
mouse model is increasingly valuable for the understanding
of human biology and disease. Recent advancements
in genomics, including the availability of complete
genome sequences for human and mouse, have made approaches
even more powerful. This mini-symposium will review
state-of-the-art methodologies in the context of developmental
biology, genetics, and genomics of the mouse.
| Program |
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| Neal
G. Copeland, NCI |
Recombineering
Provides a Powerful New Tool for Functional Genomic
Studies in Mouse |
| William
J. Pavan, NHGRI |
Functional
Genomic Analysis of Neural Crest Development |
| Mark
Lewandoski, NCI |
Controlling Gene Expression
in Mice with DNA Recombinases |
| Minoru
S. H. Ko, NIA |
Application of Mouse Embryonic
cDNA Microarrays to Stem Cell Biology |
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Molecular
Targets in Cancer
Chaired by Allan M. Weissman, NCI
11:00 a.m. to 12:30 p.m.
Natcher
Conference Center
Conference Room E1/E2
The
ability to target proteins whose expression, structure,
or cellular function is altered in tumors provides
the opportunity to vastly expand the potential to
specifically attack cancer cells. This session will
focus on exciting ways in which molecular targeting
of cancer cell proteins are being evaluated and applied
using both small molecules and antibodies.
| Program |
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| Ira
Pastan, NCI |
Immunotoxin
Therapy of Cancer |
| Len
Neckers, NCI |
Molecular
chaperones as novel anti-cancer targets: Lessons
from geldanamycin and HSP90 |
| Karen
Vousden, NCI |
Reactivation of p53 by Inhibition
of Mdm2 Ubiquitin Protein Ligase Activity |
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Neural
Immune Factors in Neurocognitive Effects and Toxicity
of Chemotherapy
Chaired by Howard A. Fine, NCI/NINDS
11:00 a.m. to 12:30 p.m.
Natcher
Conference Center
Conference Room F1/F2
Neurotoxicity is a broad term that
pertains to damage to the peripheral and central nervous
system (CNS) from either environmental and/or endogenous
insults. Such insults include ionizing radiation and
chemical toxins in the case of iatrogenic neurotoxicity,
and infectious and immunologic processes in the case
of endogenous mechanisms of neurotoxicity.
As our understanding of the cellular
and molecular physiology of the CNS increases, it
has become clear that proper neurological function
requires a coordinated, and healthy functional relationship
between neurons, glia, and vascular endothelium. Thus,
different insults may affect different cell types
or physiological processes within the CNS ultimately
leading to neuronal dysfunction and loss, the common
denominator of most neurotoxic mechanisms. Thus, the
field of neurotoxicity has become an area of interest
to scientists interested not only in neuroscience,
but also infectious diseases and immune regulatory
diseases. Now with growing evidence that cytokines
and hormonal therapy can cause acute neurotoxicity,
and both radiation therapy and chemotherapy can cause
significant long term neurocognitive toxicity in both
children and adults, investigators interested in cancer
therapy have also become interested in issues related
to damage to the CNS.
This symposium will bring together
investigators who study different clinical models
of damage to the CNS in an initial attempt to stimulate
interest in a NIH-wide initiative to better understand
the molecular, cellular, and clinical mechanisms of
neurotoxicity and to begin to devise strategies to
circumvent this devastating condition.
| Program |
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| Roland Martin, NINDS |
Modulation of Immune Function
by the Antidepressant Rolipram, a Phosphodiesterase
Type 4 Inhibitor |
| Philip Tofilon, NCI |
Radiotherapy-induced CNS Toxicity:
A Dynamic Process |
| Adriana Marques, NIAID |
Autoimmunity and Lyme Disease |
| Kathy Warren, NCI |
Detecting Neurotoxicity in
Children with Brain Tumors |
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