Global Changes in Gene Expression of Barrett’s Esophagus Compared to Normal Squamous Esophagus and Gastric Cardia Tissues

Wednesday, October 10, 2012 — Poster Session II
Noon – 2:00 p.m	Natcher Conference Center, Building 45	NCI	MOLBIO-6

Authors

• P.L Hyland
• N. Hu
• M. Rotunno
• H. Su
• C. Wang
• L. Wang
• R.M. Pfeiffer
• B. Gherman
• C. Giffen
• C. Dykes
• S.M. Dawsey
• C.C. Abnet
• P. Young
• R.D. Acosta
• B.D. Cash
• P.R Taylor

Abstract

Barrett’s esophagus (BE) is the recognized precursor lesion to esophageal adenocarcinoma (EA) and increases the risk of EA by 11 times compared to that of the general population. However, the majority of EA occurs in patients with undiagnosed BE. We sought to identify genes that are altered in BE compared to the normal esophageal mucosae, and which may be potential biomarkers of BE development or diagnosis. We performed gene expression analysis using HG-U133A Affymetrix chips on 43 fresh frozen tissues of Barrett’s metaplasia and matched normal mucosa from squamous esophagus (NE) and gastric cardia (NC). Using a cut off of 2-fold and P< 1.12E-06 (the Bonferroni corrected 0.05) for P-value, we identified 1345 differentially expressed genes comparing BE vs. NE, and 674 genes comparing BE vs. NC. We identified 262 genes whose expression was significantly altered in both BE vs. NE and BE vs. NC and showed overrepresentation in different pathways including TGF-β and Notch. Our findings provide additional data on the global transcriptome in BE tissues compared to matched normal esophageal mucosae, which should promote further understanding of gene functions and regulatory mechanisms involved in BE development, as well as insight into potential biomarkers for the diagnosis of BE.

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