PET imaging of angiogenesis after myocardial infarction/reperfusion using a one-step labeled integrin targeted tracer 18F-AlF-NOTA-PRGD2

Thursday, October 11, 2012 — Poster Session III
10:00 a.m. – Noon	Natcher Conference Center, Building 45	NIBIB	IMAG-9

Authors

• H Gao
• L Lang
• N Guo
• Q Quan
• K.O Kiesewetter
• G Niu
• X Chen

Abstract

Objective: The αvβ3 integrin represents a potential target for noninvasive imaging of angiogenesis. The purpose of this study was to evaluate a novel one-step labeled integrin αvβ3 targeting PET probe, 18F-AlF-NOTA-PRGD2, for angiogenesis imaging in a myocardial infract/reperfusion (MI/R) animal model. Methods: Male SD rats underwent 45 min transient left coronary artery occlusion followed by reperfusion. In vivo PET imaging were used to determine myocardial uptake of 18F-AlF-NOTA-PRGD2 at different time points following reperfusion. Ex vivo autoradiographic analysis and CD31/CD61 double immunofluoresence staining were performed to validate the PET results. Results: Myocardial origin of the 18F-AlF-NOTA-PRGD2 accumulation was confirmed by 18F-FDG and autoradiography. PET imaging demonstrated increased focal accumulation of 18F-AlF-NOTA-RGD2 in the infarct area started at day 3, peaked between 1 and 3 weeks. The focal accumulation decreased but still kept at a higher level than sham group after 4 months of reperfusion. Autoradiographic imaging showed the same trend of uptake in myocardial infarction area. Conclusion: PET imaging using the one-step labeled 18F-AlF-NOTA-PRGD2 allows non-invasive visualization of ischemia-reperfusion induced myocardial angiogenesis longitudinally. The favorable in vivo performance and easy production method of this integrin targeted PET tracer facilitates its future clinical translation for lesion evaluation and therapy response monitoring.

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