Speed Congenics Services at Frederick National Laboratory for Cancer Reserach

Thursday, October 11, 2012 — Poster Session III
10:00 a.m. – Noon	Natcher Conference Center, Building 45	NCI	GEN/GENOM-24

Authors

• M.C. Tseng
• W.T. Hsieh
• Lione Feigenbaum

Abstract

The genetic background of the host animal exerts great influence on the tumor frequency and the resultant latency of a particular transgene/mutation as well as the systemic host immune responses. Therefore, it is important to study the genetic modifications in a congenic mouse, which has a homogeneous genome, varying only by the region containing the transgene or targeted mutation. The congenic mouse strains are traditionally made by backcrossing random carriers to the desired inbred strain. This process can take up to 10 generations of backcrossing and 3 years to complete. The Laboratory Animal Sciences Program (LASP) at Frederick National Laboratory for Cancer Research offers to NIH/NCI-intramural investigators the custom production of congenic strains by accelerated, marker-assisted backcrossing (“speed congenics”). The speed congenics approach, which is based on selection of genetically optimal breeders at each backcross generation, allows for congenic strains to be derived in 5 generations of backcrossing and shortened the time needed to 12-14 months.

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