Identifying Cell line mutations with the Ion Torrent Ampliseq cancer panel

Thursday, October 11, 2012 — Poster Session III
10:00 a.m. – Noon	Natcher Conference Center, Building 45	NCI	GEN/GENOM-23

Authors

• D.R. Soppet
• C.C. Stewart
• J.W. Mitchell
• J.L. Troyer
• T.R. Hartley
• M.R. Spencer
• K.M. Pike
• T.M. Plona
• V. Grinberg
• N.L. Shraeder
• M.W. Smith

Abstract

Next generation sequencing technologies provide powerful tools for characterizing mutations in genes that impact the development of cancer. For clinical applications rapid sequencing to high coverage depth for targeted regions can provide reliable, sensitive and cost effective identification of cancer related mutations. The Ion Torrent personal Genome Machine uses semiconductor technology based detection of hydrogen ion release to generate rapid cost effective sequence. The AmpliSeq Cancer panel enables generation of Ion Torrent ready sequencing libraries for a set of cancer genes in a few hours from as little as 10 ng of input genomic DNA. We have implemented Ampliseq library preparation and Ion Torrent sequencing in our laboratory and demonstrate its capabilities by identifying mutations in a set of well characterized cell lines

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