Knockouts for half of all mammalian genes are available from the KOMP repository: http://www.komp.org/

Thursday, October 11, 2012 — Poster Session III
10:00 a.m. – Noon	Natcher Conference Center, Building 45	OD	GEN/GENOM-18

Authors

• R.R. O'Neill
• P. de Jong
• K.C. Lloyd

Abstract

NIH and CBER investigators can purchase >8,500 different mouse ES cell lines carrying specific gene knockouts for $1470 for the first clone, and $805 each for additional clones of the same target (http://komp.org/fees.php). Each clone is mycoplasma /mouse parvovirus-free. >10,700 KO vectors for targeting recombination in ES cells, and >745 KO lines of mice/sperm are available. ~300 mouse strains have been produced and their phenotypic data is available (http://www.kompphenotype.org). Many of the KO genes have annotations in http://www.ncbi.nlm.nih.gov/omim/ (OMIM) which can be accessed through the Advanced Search page (http://www.komp.org/advancedSearch.php. Many of the KOed genes may be embryonic lethals when homozygous. Several vector constructs (diagrammed at http://komp.org/alleles.php#deletion-csd) are used, and conditional vectors allow tissue-specific or cell-type-specific perturbations by temporally or spatially restricting expression of the KO vector phenotype via combination with various patterns of Cre-recombinase expression. Funding of KOMP2 from 2011-2016 will convert the IKMC knockout ES cell libraries into 2,500 lines of mice that have comprehensive phenotypic data. KOMP2 has co-funding from many NIH ICs and the Common Fund. http://www.komp.org/ will be the source to obtain mice or sperm, and the web site http://mousephenotype.org/ will provide access to the comprehensive phenotypic data and protocols.

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