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Knockouts for half of all mammalian genes are available from the KOMP repository:

Thursday, October 11, 2012 — Poster Session III

10:00 a.m. – Noon

Natcher Conference Center, Building 45




  • R.R. O'Neill
  • P. de Jong
  • K.C. Lloyd


NIH and CBER investigators can purchase >8,500 different mouse ES cell lines carrying specific gene knockouts for $1470 for the first clone, and $805 each for additional clones of the same target ( Each clone is mycoplasma /mouse parvovirus-free. >10,700 KO vectors for targeting recombination in ES cells, and >745 KO lines of mice/sperm are available. ~300 mouse strains have been produced and their phenotypic data is available ( Many of the KO genes have annotations in (OMIM) which can be accessed through the Advanced Search page ( Many of the KOed genes may be embryonic lethals when homozygous. Several vector constructs (diagrammed at are used, and conditional vectors allow tissue-specific or cell-type-specific perturbations by temporally or spatially restricting expression of the KO vector phenotype via combination with various patterns of Cre-recombinase expression. Funding of KOMP2 from 2011-2016 will convert the IKMC knockout ES cell libraries into 2,500 lines of mice that have comprehensive phenotypic data. KOMP2 has co-funding from many NIH ICs and the Common Fund. will be the source to obtain mice or sperm, and the web site will provide access to the comprehensive phenotypic data and protocols.

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