Flow Cytometry sorting of Dendritic Cells engaged in Endocytosis of Albumin labeled with Fluorescein

Thursday, October 11, 2012 — NIH Core Poster Session
10:00 a.m. – Noon	Building 10 South Lobby	NEI	CORE-9

Authors

• RS Villasmil
• P Chen
• CA Jeffries
• B Liu
• RB Nussenblatt

Abstract

Dendritic cells (DCs) are an important part of the mammalian immune system. DCs process antigen material and function as antigen-presenting cells. DCs have a role in acquired and autoimmune diseases, allergies, and other health issues. Endocytosis is a process by which DCs uptake antigens. Two types of DCs have been identified based on their endocytosis function: high and low antigen uptake capacity. Here, we describe a method of identification and isolation of high and low antigen uptake based on flow cytometry. This method measures DC uptake of albumin labeled with Fluorescein Isothiocyanate (FITC). Two populations of live DCs were identified by their fluorescent dye uptake and isolated. These cells are provided to users of the National Eye Institute Flow Cytometry Core for further study of DC function. Flow cytometry sorting of dendritic cells engaged in endocytosis of albumin labeled with fluorescein is a useful tool to isolate subgroups of DCs by their endocytosis function.

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