Circulating miRNAs and interleukin-10 levels in patients with and without chronic abdominal pain.

Wednesday, October 10, 2012 — Poster Session II
Noon – 2:00 p.m	Natcher Conference Center, Building 45	NINR	CLIN/TRANS-10

Authors

• W.A. Henderson
• R.M. Peace
• N. Fourie
• A.C. Martino
• A.Y. Del Valle-Pinero
• B.L. Majors
• D. Wang
• S. Reddy

Abstract

Chronic abdominal pain (CAP) of unknown origin affects 15-20% of people world-wide. Predictive biomarkers are needed to improve assessment of CAP. Dysregulation of Interleukin-10 (IL-10) is suspected in CAP. This study tested the expression of circulating IL-10 and correlating miRNA in patients with and without CAP. Blood was collected from 21 CAP patients and 33 healthy controls. IL-10 protein levels were measured using immunoassay. RNA was extracted and quantified via spectrophotometry. In a subgroup of twenty gender, age, race matched samples (10 CAP/10 controls) miRNA PCR array was performed followed by digital detection and quantification of 735 miRNAs. IL-10 protein levels were significantly higher in CAP (3.18 ± 0.68 pg/mL) compared to controls (1.35 ± 0.20 pg/mL). Patients with CAP were 2.4 times more likely to have higher IL-10 levels. 340 miRNA were identified via the random variance estimation procedure of which 320 passed filtering criteria. Multiplex targeted profiling revealed eight miRNA that had parametric significance at p ≤.05 and strong correlations with the IL-10 PCR array data. These findings indicate interleukin dysregulation at both the protein and molecular level, and suggest novel diagnostic markers of CAP, and point to potential treatment targets.

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