Development of a sensitive microarray immunoassay for the quantitative analysis of Neuropeptide Y

Wednesday, October 10, 2012 — Poster Session II
Noon – 2:00 p.m	Natcher Conference Center, Building 45	NIMH	CHEM-6

Authors

• M Jia
• E Belyavskaya
• P Deuster
• E.M. Sternberg

Abstract

A direct competitive immunoassay in an antibody microarray format was developed for the sensitive detection of neuropeptide Y (NPY) in human sweat samples. This is the first demonstration that antibody microarray, as a powerful multiplex analysis tool, can be used for the sensitive determination of NPY and potentially other neuropeptides in human sweat samples. Under optimal conditions, a sensitivity of 50 pg/ml and a linear dynamic range of 0.1-100 ng/ml for NPY were obtained. Evaluation of matrix effects by using artificial sweat revealed that dialysis is necessary for analyzing NPY in human sweat samples with microarray immunoassay. 0-240 pg/ml of NPY was detected in sweat samples collected with Macroduct collectors. This study indicates that antibody microarrays can be used for NPY analysis and that human sweat could be a valuable sample source for biomarker and proteomics studies, especially when non-invasive human sample collection is preferable.

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