Genes related to mitochondrial biogenesis and bioenergetics are associated with fatigue in men with prostate cancer receiving external beam radiation therapy

Wednesday, October 10, 2012 — Poster Session II
Noon – 2:00 p.m	Natcher Conference Center, Building 45	NINR	CANCER-13

Authors

• CP Hsiao
• D Wang
• A Kaushal
• L Saligan

Abstract

PURPOSE: This prospective study explored relationships between changes in expression of genes related to mitochondrial biogenesis and bioenergetics and fatigue in prostate cancer patients before, at midpoint, and at completion of external beam radiation therapy (EBRT). METHODS: Fatigue was measured using the 7-item Patient Reported Outcomes Measurement Information System-Fatigue short form (PROMIS-F). RNA from whole blood samples was collected using PAXgene tubes. The Human Mitochondria RT2 Profiler™ PCR Array system was used to identify differential expression of genes. Generalized estimating equation (GEE) model was fit to determine the association of gene expression with fatigue score over time. RESULTS: Twenty five subjects scheduled for EBRT and 25 race and age-matched prostate cancer patients on active surveillance were enrolled. Mean fatigue T-scores were 44.87 + 5.89 at baseline, 49.02 + 5.29 at midpoint, and 48.22 + 6.84 at completion. Fourteen genes related to mitochondrial integrity, apoptosis, molecular transport, and the respiratory chain were differentially expressed. BCL2L1 (β= -1.68, P=0.003), BCS1L (β=1.30, P=0.002), FIS1 (β=-2.35, P=0.001) and SLC25A37 (β=-2.44, P=0.001) were significantly associated with the changes in fatigue overtime during EBRT. CONCLUSIONS: Genes related to mitochondrial membrane integrity and energy production were associated with increased fatigue experienced by subjects during EBRT.

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