Surface plasmon resonance (SPR) biosensor study of biomolecular interactions

Tuesday, October 09, 2012 — Poster Session I
1:00 p.m. – 3:00 p.m	Natcher Conference Center, Building 45	NIBIB	BIOPHY-2

Author

• I. Gorshkova

Abstract

Optical biosensor studies based on Surface Plasmon Resonance (SPR) have become one of the most important tools for examining molecular interactions, following the introduction of instruments such as those produced by Biacore (Upsala, Sweden) in the early nineties. Even today, no comparable technique exists to characterize molecular interactions in real time, without molecular labeling, and in an automated and robust fashion. SPR biosensors are used in either qualitative mode to determine binding parameters or quantitative mode to determine the kinetics and affinity parameters of an interaction. Traditionally, most applications describe interactions where both binding partners are proteins, i.e. protein – protein, antigen – antibody, or peptide – receptor. A smaller fraction of the SPR literature describes interaction of proteins with nucleic acids, small molecules (e.g., drug candidates), or lipid membranes, etc. The purpose of this poster is to present the basics of the SPR technique and its application to diverse biological systems, and to advertise this technique in the hope of fostering future collaborations.

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