October 9 - 12, 2012
Building 10 & Natcher Conference Center
- General Schedule of Events
- Opening Plenary Session
- Concurrent Symposia Sessions
- National Graduate Student Research Conference
- FARE Award Ceremony
- Poster Sessions
- Special Exhibits on Resources for Intramural Research
- TSA Research Festival Exhibit Show
- Research Festival Committees
- Past Research Festivals
2011 program
Download the 2011 Research Festival Program Book
PDF documents require the free Adobe Reader
SAGE: A browser based software for rapidly analyzing and archiving qPCR data
| Tuesday, October 09, 2012 — Poster Session I | |||
|---|---|---|---|
1:00 p.m. – 3:00 p.m |
Natcher Conference Center, Building 45 |
NIAID |
BIOINFO-20 |
Authors
- Q. Tan
- T.R. Ramalingam
- Y. Guo
- Y. Huyen
Abstract
SAGE (Simplified Analysis of Gene Expression) is designed to parse raw, ungrouped data exported from real time qPCR instruments and quickly analyze relative gene expression values based on user supplied information. In short, the user inputs their sample template, replicate information, list of genes, and *.txt raw data file. SAGE then analyzes the gene expression data, calculates the difference in cycle threshold (dCT) by using the comparative CT method, and provides the user with relative quantitation of gene expression (fold changes) for each experimental sample. Further, SAGE provides immediate graphical results and downloadable data from current or previously analyzed and archived experiments.
