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Identifying stable reference genes for RT qPCR with hkgFinder

Tuesday, October 09, 2012 — Poster Session I

1:00 p.m. – 3:00 p.m

Natcher Conference Center, Building 45




  • J.R. Skinner
  • Q.Q. Li
  • D.T. Liou
  • J.E. Bennett
  • Y. Huyen


Real-time quantitative PCR (RT qPCR) is a popular method for researchers seeking precise measurements of differential gene expression, but reliable results from RT qPCR require the choice of one or more appropriate reference genes. The hkgFinder Web tool provides an easy-to-use resource for the identification of stable reference genes from RT qPCR data using standard deviations and calculations of the fold change between two phenotypes. After one or more reference genes are selected, Student’s T-tests are computed to estimate differential expression for the remaining genes. Results from hkgFinder are compared to results from three other popular tools (geNorm, BestKeeper and NormFinder) for azole-stimulated Candida glabrata RT qPCR data.

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